Yeast-based solutions for sustainable Aviation Fuels (YAF) aims transforming industrial biowaste into sustainable biofuels through precision fermentation. The consortium comprises of six members, Tallinn University of Technology being one of them. TalTech Bioengineering team´s expertise includes:
- Bio-conversion of residual carbon sources into mono-terpenes using R. toruloides. Development of new tools for the metabolic engineering. Establishment of rapid screening of terpene producing strains using (FTIR) platform.
- Enzyme design towards more efficient production of mono-terpenes used in SAFs. Development of bioinformatics tools for enzyme design. Automatization of metabolic engineering and enzyme design and engineering
- Development of new tools to accelerate the production of sustainable aviation fuels (SAF) in Y. lipolytica by: 1) Development of a multiplexed CRISPR-based toolbox which makes strain improvement 2-5 times faster. 2) Use genome-scale models to predict targets for the multiplexed technology to redirect metabolic fluxes toward SAF precursors. 3) Use the toolbox to accumulate either terpenes or fatty acids.
More information can be found from the project website.