Author: Angelika Sofia Bolkvadze

People
BioEng Lab & ÄIO International Picnic

Before the academic year begins and summer loses its warmth, the BioEng Lab and ÄIO teams decided to have a common lunch in the format of an international picnic. Everyone was invited to bring something representing their country’s cuisine or culture. The table soon filled up with a variety of savoury dishes, sweet cakes and snacks, offering plenty of opportunities to discover something new. 

The biggest surprise of the event, however, was the grill that appeared from the ÄIO’s storage room. Soon, the smell of meat in a Brazilian marinade was filling the Tehnopol air, making it rather difficult to remember that this was supposed to be just a lunch break. 

Instead, it turned into a relaxed afternoon with good food, conversations and plenty of fun — a simple reminder that collaboration can also happen away from desks, meetings and laboratories. 

The same activity next year? 

EventPeople
Summer Day 2026

What´s the better way to enjoy Estonia’s beautiful nature than to escape to one of its islands for a day? This year, the BioEng team decided to explore Aegna island — a place that might surprise you with one interesting fact: although it feels like a remote island getaway, Aegna is actually part of the city of Tallinn! 

Our adventure began on the 17th of July at Tallinn’s Linnahall harbor, where we boarded the ferry. The ferry, which can accommodate around 100 passengers, was packed, with every ticket sold. Most of the passengers were Estonians, which seemed to be a great example of how popular domestic tourism is among locals. 

On the island, our guide was waiting to welcome us, and our adventure began. Our first stops took us into the island’s fascinating history. We explored several places, including the Officers’ Casino and the remains of Peter the Great’s Naval Fortress — more specifically, the “Heavy Batteries of the Tallinn–Porkkala Line”. Walking among these remnants of the past, it was easy to imagine how strategically important this seemingly peaceful island once was. 

After exploring the historical sites, we made our way towards the beach. We found a perfect spot in the shade of the trees and settled down for a picnic. Some brave members of the team dipped their toes into the sea, but despite it being summer, the water was surprisingly ice-cold! 

With renewed energy after lunch, we headed towards one of Aegna’s most mysterious attractions — its magical stone labyrinth. The 11-turn stone labyrinth, also known as “Jerusalem”, is an important part of Aegna’s cultural heritage. Throughout history, labyrinths have been associated with rituals, mystery, and spiritual journeys. This one is said to have been regarded as a ritual sanctuary and a place for connecting with supernatural forces. One of the BioEng members even completed the entire labyrinth — but whether it really has a magical effect remains a secret. 

Our next destination was the impressive Tulekivi boulder. In the past, a fire was lit on top of it to serve as a navigational signal for ships approaching the island. On this day, however, the boulder had a different purpose — it was conquered by the BioEng team! The climb up was surprisingly easy, although getting back down proved to be a little more complicated. 

Our adventure continued with a visit to a boulder field before we followed a magical trail marked by a continuous line of stones. Here, we discovered another fascinating island tradition: everyone who walks the trail is encouraged to pick up a stone from the shore and add it to the markings along the path, making the trail a little longer for the next visitors. In this way, every person who visits becomes a small part of the island’s story. 

Eventually, following the trail, we made our way back to the port. Before boarding the ferry, there was, of course, one final and essential stop: the ice-cream kiosk! As the ferry carried us back towards Tallinn, we could look back on a day filled with sunshine, history, nature, adventure and plenty of laughter. But the day was not over yet. Back in Tallinn, we continued our summer adventure with a delicious Georgian meal. 

And so, with tired legs, happy faces and plenty of new memories, we brought our amazing BioEng Summer Day to an end.

 

(Thank you Adventurehiking OÜ for an amazing guide Jaan Jalas)

NetworkingPeopleProjects
Mid-term Meeting and Workshop by YAF (Yeast-based Solutions for Sustainable Aviation Fuels)

Abdullah Nasir, Subhasis Behera and Laura de Freitas Naves from our BioEng research group recently participated in the two events by YAF (Yeast-based Solutions for Sustainable Aviation Fuels) project.

Mid-term Meeting 

Meeting held at CIEMAT, and coordinated by IMDEA Energía Energy, in Madrid. 

During the meeting, each Doctoral Candidate presented the main achievements and activities developed within their Individual Research Projects since the last meeting, showcasing the excellent progress being made across the programme. These sessions provided valuable discussions, feedback, and new perspectives, reinforcing the collaborative spirit of the project.  

Biorefinery for Biofuels & Chemicals Training 

The training was held in Turku, Finland. The three-day training provided an in-depth overview of modern biorefinery concepts and sustainable biomass valorization, covering topics such as lignocellulosic biomass processing, thermochemical conversion technologies, algae-based biorefineries, bioactive compounds, and industrial case studies from pilot-scale biorefineries.  

The workshop fostered interdisciplinary learning and collaboration while strengthening expertise in circular bioeconomy and sustainable production of biofuels and biochemicals.

 

 

 

 

 

 

 

 

EventNetworking
Machine Learning for Biosciences

PhD student Abdullah Nasir from the BioEng Lab research group participated in the 9th BioSpec Interdisciplinary Summer School in Machine Learning for Biosciences, organised by the Norwegian University of Life Sciences (NMBU). 

The aim of the summer school was to introduce the participants to the analysis of biological data using infrared spectroscopy and machine learning. Through lectures and a hands-on interdisciplinary project, it provided practical skills in FTIR data processing, spectral pre-treatment, multivariate analysis and machine-learning methods, while strengthening the ability to tackle complex bioscience problems collaboratively.  

These skills and insights are directly relevant to research involving biological samples and spectroscopic data. 

Defence
Oksana Tingajeva defended her MSc thesis

Congratulations!
Oksana Tingajeva, an MSc student in the Applied Chemistry and Biotechnology curriculum, has successfully defended her thesis “Rhodotorula toruloides bioprocess analysis for biopolymer production”.

Supervisor: Rahul Kumar, Senior Researcher – Department of Chemistry and Biotechnology
Co – supervisor: Henrique Sepulveda Del Rio Hamacek ,PhD student & Early Stage Researcher – Division of Food and biotechnology: Department of Chemistry and Biotechnology
Reviewer: Kaarel Adamberg, Senior Researcher – Division of Food and biotechnology: Department of Chemistry and Biotechnology

(Summary of the thesis)
Although Rhodotorula toruloides is widely studied for lipid production, its potential for exopolysaccharide (EPS) synthesis in bioreactor conditions remains underexplored. This study investigated how cultivation parameters—such as pH control, nutrient composition, and osmotic stress—affect biomass formation, EPS production, and cell morphology.

Bioreactor cultivations showed that buffered, pH-controlled, and urea-supplemented conditions significantly increased biomass (≈14–20 g L⁻¹), while control and high-salt conditions resulted in lower biomass (≈6–7 g L⁻¹). EPS production was highest under control and buffered conditions (≈3–4 g L⁻¹), with minimal production under other conditions. Protein content and morphology varied, with filamentous and aggregated structures observed under certain conditions, highlighting the influence of pH and nutrient balance on cellular adaptation.

Overall, the results demonstrate that cultivation conditions strongly impact biomass composition and EPS synthesis in R. toruloides, supporting its potential as a versatile platform for biopolymer production. Further research should focus on process optimization and molecular-level analyses to better understand EPS biosynthesis and carbon allocation.

This work provides a strong foundation for a planned scientific publication. 📄
We look forward to seeing where your passion for science will take you next! 🔬

GeneralProjects
1st place – Nucleate Baltics Genesis Demo Day 2026 at Deep Tech Atelier 2026

A special congratulations to our PhD student Subhasis Behera´s team BeeYOND for achieving 1st place in the competition – Nucleate Baltics Genesis Demo Day 2026 at Deep Tech Atelier 2026! 

Over the past months, talented and passionate innovators from across Latvia, Estonia, and Lithuania came together to transform scientific ideas into impactful biotech and deep tech solutions.  

BeeYOND idea was to create honey without bees—while preserving the same nutritional and functional benefits. Using locally sourced ingredients and precision fermentation, the team aims to produce a bee-free honey rich in enzymes and antioxidants.  

Wild honeybees are increasingly at risk of extinction across the EU, raising concerns about the long-term sustainability of traditional honey production. At the same time, the European honey market is projected to grow by 4.8% by 2030, driven by rising awareness of honey’s health benefits, including immune system support. Consumers are also shifting toward natural sweeteners over synthetic alternatives. 

Currently, local honey production meets only about 63% of demand, while up to 46% of imported honey is suspected to be adulterated. BeeYOND addresses both sustainability and quality challenges by offering a reliable and innovative alternative to conventional honey.  

“We need a sustainable source of honey without risking the honey-bee popluation.” 

 

Looking forward to tasting “Bee-Free-Honey” in the future! 

Defence
Andrei Jepifanov defended his MSc thesis

Congratulations! 
Andrei Jepifanov, an MSc student in the Applied Chemistry and Biotechnology curriculum, has successfully defended his thesis 
“Metabolic engineering strategies to address limiting xylose uptake in Rhodotorula toruloides“. 
 
SupervisorJuliano Sabedotti De Biaggi, Institute of Chemistry and Biotechnology, Researcher 
Reviewer: Evely Ojangu, Institute of Chemistry and Biotechnology, Engineer 
 
(Summary of the thesis) 
The non-conventional yeast Rhodotorula toruloides is a promising microbial cell factory due to its tolerance to harsh conditions and ability to utilize xylose—a key sugar in wood residues. Improving xylose metabolism could support more sustainable bioprocessing, although its efficiency still lags behind glucose. 
 
This study aimed to enhance growth on xylose by introducing heterologous pathway genes from Candida intermedia, Scheffersomyces stipitis, and Escherichia coli. Transformants were generated using the Lithium Acetate method and screened for growth performance, with gene insertion confirmed by PCR. 
 
While gene integration was successful, no significant improvement in xylose-based growth was observed. Variability among strains was likely due to random genomic integration via non-homologous end joining (NHEJ), as confirmed by PCR. 
 
Further research is needed to evaluate gene expression, analyze byproducts, and explore additional or combined pathway genes to improve xylose utilization and cell growth. 
 
We wish you exciting discoveries and continued success in your future projects!