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EventProjects
Estonian Bioeconomy conference
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Collaboration project between TalTech, University of Tartu and Life-science University together with three Estonian ministries  – ADDVAL-BIOEC – Added value in Estonian bioeconomy – with a strong contribution also from our Bioengineering group is finishing. The collected research will be presented at the conference on October 26th, 2021, at Erinevate Tubade Klubi, Telliskivi 60-A1, Tallinn (event language is Estonian).

 

More information: www.taltech.ee/biomajandus

Register to the event: https://forms.gle/hiUc9MCBefLsPePi8

Event
Guest seminar by Sergio Bordel
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You are welcome to participate in the following guest seminar.

 

Prof. Sergio Bordel Velasco

Department of Chemical Engineering and Environmental Technology, University of Valladolid, Spain

Title: Metabolism of methanotrophic bacteria. A Genome-Scale modeling perspective.

When: Thursday, October 14th at 4 PM

Where: SCI-028 (on-line link will follow)

 

Link to publications: https://scholar.google.com/citations?hl=en&user=I2K5LLUAAAAJ

Host: Assoc. Prof. Petri-Jaan Lahtvee

Projects
Yeast4Bio Training School
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On September 13-17h happened the first Yeast4Bio Training School financed by the COST agency and  coordinated by Jose L. Martinez (Associate Professor and Head of Fermentation core in the Technical University of Denmark). Our group member, Gintarė Liudžiūtė learned there fundamental knowledge of fermentation design and analysis applied to non-conventional yeasts which are used as cell factories for high-value compounds production. Attending to the first Yeast4Bio Training School was a significant personal and professional opportunity to develop scientific competences into our group.

EventProjects
Yeast4Bio COST Action meeting in A Coruna, Spain
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It was a great pleasure to visit the COST Action Yeast4Bio bi-annual conference in A Coruna, Spain and chair a session on non-conventional yeast Systems and Synthetic Biology together with Prof. Rodrigo Ledesma-Amaro.

Two days full of exciting scientific discussions!!! Thank you to all the speakers and participants for creating such a nice environment and Prof. Christian Kennes @UDC_gal for organising it. #bioeconomy #nonconventionalyeast #bioproducts

More info on Yeast4bio: https://yeast4bio.eu/
Event
Seminar
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On the 28th of October 2021 (14h- 16h) a seminar will be given by Petri-Jaan Lahtvee (Assoc. Professor) on Circular Economy at ALU Freiburg (Germany), one of the partner in the Local Electron Delivery in Bioinks for Efficient Yeast Cell Factories project. 

This project of the Baltic-German University Liaison Office is supported by the German Academic Exchange Service (DAAD) with funds from the Foreign Office of the Federal Republic Germany.

 

Here the link to access the conference

https://uni-freiburg.zoom.us/j/66501878693?pwd=Sk1WQk1HNXpIdlBIRjhJWGlSYVB0QT09

 

People
New students
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Two new students have joined the group. Supported by Dora Plus scholarship both PhD students from Brazil, Luísa Czamanski and Julianno Sabedotti De Biaggi, will work 6 and 3 months on R. toruloides, respectively. Luísa will focus on engineering R. toruloides strains capable of producing terpenes, which have a wide range of applications such as precursors of renewable fuels. Julianno will focus on R. toruloides carotenoid metabolism by expressing the enzyme phytoene desaturase from multiple phyla of Fungi in Escherichia coli and analysing its activity on different substrates in vitro.

People
New student
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 Artjom Tšitšerin, a high school student at Tallinn Central Russian Gymnasium, has joined our group to curate the carotenoid pathway by investigating whether overexpressing an heterologous xylulokinase in R. Toruloides could improve carotenoid yield.
Publications
New manuscript on 3D printing of living materials
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We are excited to announce a new publication on 3D printing of living materials. In this ACS – Applied Bio Materials publication led by Tobias Butelmann, we examined the permissibility of oxygen in hydrogels and how yeast metabolism functions in response to immobilization.

This publication results from our TalTech research group’s ongoing fruitful collaboration with the Alshakim Nelson’s Laboratory  at University of Washington and Tarmo Tamm’s Laboratory at Tartu University.

Congrats to all co-authors for the fantastic achievement!

 

 

 

 

 

 

 

Congrats to all the authors for their great work!

Event
Bioengineering lab presentations at ICY15
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Prof. Petri Lahtvee and senior scientist Dr. Nemailla Bonturi were presenting at this year’s largest yeast conference ICY15 meets ICYGMB 30 (International Conference of Yeast meets International Conference on Yeast Genetics and Molecular Biology), taking place on August 23-27, 2021 and hosted by BOKU, Austria.

Petri gave a talk and chaired the Systems Biology session, while Nemailla gave a talk at the Synthetic Biology session.

More info: https://icy15.boku.ac.at/

Event
Upcoming Guest Seminar about Living Materials
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During his research visit at TalTech, our partner from ALU Freiburg, Tobias Butelmann, will give a seminar (seminar room SCI-109 or online here) about living materials and the current project about Local Electron Delivery in Bioinks for Efficient Yeast Cell Factories. The seminar will be publicly available. Since this project is highly interdisciplinary, we invite all interested people to join this seminar!

 

Date: 06 August 2021, 11:00 – 12:00.

 

 

Abstract: The need for sustainable processes to produce value-added chemicals and materials displays a major challenge of our time. Engineered microbes have the proven power to solve these challenges, as more efficient processes are required in terms of the cell factory as well as the cultivation performance. Bioprocesses currently used in industry are primarily based on batch and fed-batch mode, where yields and productivities have peaked and require technological innovations to uplift this cap on productivities. Additive manufacturing technologies can provide opportunities to move beyond traditional processes and empower a new industrial revolution. 3D printing can be applied together with engineered microbes to print living materials with superior properties to common suspension cultures used in biorefineries. Those living materials consist of a bioink that encapsulates the host organism – a cell factory for the desired product.

One bottleneck in engineered microbes is the regeneration of co-factors, which are critical for their efficiency. To overcome this, special conductible bioinks for living materials can be designed that help embedded microbes by the delivery of electrons for co-factor regeneration. The successful implementation of such a platform has the potential to increase the efficiency of engineered microbes by multiples and, hence, create sustainable biorefinery processes.

 

This project of the Baltic-German University Liaison Office is supported by the German Academic Exchange Service (DAAD) with funds from the Foreign Office of the Federal Republic Germany.

 

Publications
New manuscript on automated cell size measurement
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The work of our former BSc student Aleksandr Illarianov was recently published in Applied and Environmental Microbiology journal, where he developed a machine learning algorithm for the analysis of optical microscopy images. The algorithm was applied for the analysis of more than 500 000 cells in a comparative study of conventional and non-conventional yeast stress responses.

Congrats to all the authors for their great work!

 

Link to the manuscript: https://doi.org/10.1128/AEM.03100-20

        SCImago Journal & Country Rank

Projects
3D printing project
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Tobias Butelmann, a former member (2018-2019) of Petris’ group will be visiting us next August. The collaboration entitled Local Electron Delivery in Bioinks for Efficient Yeast Cell Factories will provide valuable knowledge and inputs for 3D printing project. This project of the Baltic-German University Liaison Office is supported by the German Academic Exchange Service (DAAD) with funds from the Foreign Office of the Federal Republic Germany.

 

Projects
New projects started – Perfecoat and SAFE
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We are excited to announce the start of two interdisciplinary international projects PERFECOAT – High performance bio-based Functional coatings for wood and decorative applications – with funding from Horizon2002 Bio-Based Industries Joint Undertaking; and SAFE – Sustainable Aquaculture Feed Based on Novel Biomass from Wood By-products, funded by NordForsk.

                 

Publications
New publication is out now!
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We have a new publication “Screening and growth characterization of non-conventional yeasts in a hemicellulosic hydrolysate” published in Frontiers in Bioengineering and Biotechnology. Here we have characterized the consumption profiles and growth patterns of nine non-Saccharomyces yeast strains on an industrial hemicellulosic hydrolysate, provided by our partners from Graanul Biotech.

 

Congrats to all the authors Paola, Daria, Nemailla and Petri.

 

For full text, please click here.

General
Thesis graduations in 2021
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Next Friday (4th of June) Alina Rekena will defend her Master’s degree at the University of Tartu. Alina carried out a large-scale study characterizing R. toruloides growth on different substrates and creating enzyme constrained genome-scale metabolic models. Her work will be a keystone to engineer carotenoid and lipid yields in R. toruloides. 

 

 

Daria defended her bachelor’s thesis “Characterisation of non-conventional yeast Kluyveromyces marxianus in lignocellulosic hydrolysate” on 31st of May with the highest grade.

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